Western blotting analysis using growth associated protein 43 antibody (Cat#4177). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with growth associated protein 43 antibody (Cat#4177, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with Growth associated protein 43 antibody (Cat#4177, 1:1,000). Nuclei were stained blue with DAPI; Growth associated protein 43 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Immunohistochemistry was performed on paraffin-embedded human glioblastoma using growth associated protein 43 antibody (Cat#4177, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Applications Tested: Western blotting (WB), immunohistochemistry-paraffin (IHC-P)
Immunogen
A synthesized peptide derived from human GAP43
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:2,000-1:10,000 Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note
This product is for research use only.
Data
Western blotting analysis using growth associated protein 43 antibody (Cat#4177). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with growth associated protein 43 antibody (Cat#4177, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with Growth associated protein 43 antibody (Cat#4177, 1:1,000). Nuclei were stained blue with DAPI; Growth associated protein 43 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Immunohistochemistry was performed on paraffin-embedded human glioblastoma using growth associated protein 43 antibody (Cat#4177, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Western blotting analysis using growth associated protein 43 antibody (Cat#4177). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with growth associated protein 43 antibody (Cat#4177, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with Growth associated protein 43 antibody (Cat#4177, 1:1,000). Nuclei were stained blue with DAPI; Growth associated protein 43 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Immunohistochemistry was performed on paraffin-embedded human glioblastoma using growth associated protein 43 antibody (Cat#4177, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.002634s ]
SELECT * FROM `goods` WHERE `code` = '4177' LIMIT 1 [ RunTime:0.000525s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000477s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.002226s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000429s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 5511 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000420s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.002713s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 5511 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.002001s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000458s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000259s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.142091s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (141,5058,5444,9386,4555,348,4768,9194,9404,5199) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.004452s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000557s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.002260s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.003358s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.003449s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000389s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000449s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.001320s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.004422s ]
