Western blotting analysis using ABCB1 antibody (Cat#4182). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ABCB1 antibody (Cat#4182, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded mouse kidney using ABCB1 antibody (Cat#4182, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Immunohistochemistry was performed on paraffin-embedded mouse liver using ABCB1 antibody (Cat#4182, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Applications Tested: Western blotting (WB), immunohistochemistry-paraffin (IHC-P)
Immunogen
A synthesized peptide derived from human P Glycoprotein
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:5,000 Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note
This product is for research use only.
Data
Western blotting analysis using ABCB1 antibody (Cat#4182). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ABCB1 antibody (Cat#4182, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded mouse kidney using ABCB1 antibody (Cat#4182, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Immunohistochemistry was performed on paraffin-embedded mouse liver using ABCB1 antibody (Cat#4182, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Western blotting analysis using ABCB1 antibody (Cat#4182). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ABCB1 antibody (Cat#4182, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded mouse kidney using ABCB1 antibody (Cat#4182, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Immunohistochemistry was performed on paraffin-embedded mouse liver using ABCB1 antibody (Cat#4182, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000943s ]
SELECT * FROM `goods` WHERE `code` = '4182' LIMIT 1 [ RunTime:0.000598s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000523s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000426s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000535s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 5516 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000438s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000529s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 5516 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000367s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000507s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000374s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.090383s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (195,10175,8120,8459,9716,7168,4084,8792,6293,9427) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001131s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000536s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000286s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000612s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000286s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000416s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000515s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000295s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000317s ]
