Western blotting analysis using cleaved Caspase-9 antibody (Cat#4188). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with cleaved Caspase-9 antibody (Cat#4188, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using cleaved caspase-9 antibody (Cat#4188). Total cell lysates (30 μg) from HeLa and HAP-1 cells untreated or treated with staurosporine (1μM, 3hr) were loaded and separated by SDS-PAGE. The blot was incubated with cleaved caspase-9 antibody (Cat#4188, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of cleaved Caspase-9 expression in HepG2 cells using cleaved Caspase-9 antibody (Cat#4188, 1:2,000). Green, isotype control; red, cleaved Caspase-9.
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
A synthesized peptide derived from human CASP9
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:200-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using cleaved Caspase-9 antibody (Cat#4188). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with cleaved Caspase-9 antibody (Cat#4188, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using cleaved caspase-9 antibody (Cat#4188). Total cell lysates (30 μg) from HeLa and HAP-1 cells untreated or treated with staurosporine (1μM, 3hr) were loaded and separated by SDS-PAGE. The blot was incubated with cleaved caspase-9 antibody (Cat#4188, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of cleaved Caspase-9 expression in HepG2 cells using cleaved Caspase-9 antibody (Cat#4188, 1:2,000). Green, isotype control; red, cleaved Caspase-9.
Western blotting analysis using cleaved Caspase-9 antibody (Cat#4188). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with cleaved Caspase-9 antibody (Cat#4188, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using cleaved caspase-9 antibody (Cat#4188). Total cell lysates (30 μg) from HeLa and HAP-1 cells untreated or treated with staurosporine (1μM, 3hr) were loaded and separated by SDS-PAGE. The blot was incubated with cleaved caspase-9 antibody (Cat#4188, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of cleaved Caspase-9 expression in HepG2 cells using cleaved Caspase-9 antibody (Cat#4188, 1:2,000). Green, isotype control; red, cleaved Caspase-9.
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