Western blotting analysis using TIA1 antibody (Cat#4316). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TIA1 antibody (Cat#4316, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of TIA1 expression in HAP-1 cells using TIA1 antibody (Cat#4316, 1:2,000). Green, isotype control; red, TIA1.
Immunocytochemical staining of HAP-1 cells with TIA1 antibody (Cat#4316, 1:1,000). Nuclei were stained blue with DAPI; TIA1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using TIA1 antibody (Cat#4316). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TIA1 antibody (Cat#4316, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of TIA1 expression in HAP-1 cells using TIA1 antibody (Cat#4316, 1:2,000). Green, isotype control; red, TIA1.
Immunocytochemical staining of HAP-1 cells with TIA1 antibody (Cat#4316, 1:1,000). Nuclei were stained blue with DAPI; TIA1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using TIA1 antibody (Cat#4316). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TIA1 antibody (Cat#4316, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of TIA1 expression in HAP-1 cells using TIA1 antibody (Cat#4316, 1:2,000). Green, isotype control; red, TIA1.
Immunocytochemical staining of HAP-1 cells with TIA1 antibody (Cat#4316, 1:1,000). Nuclei were stained blue with DAPI; TIA1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
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