Western blotting analysis using glucose-6-phosphate dehydrogenase antibody (Cat#4322). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with glucose-6-phosphate dehydrogenase antibody (Cat#4322, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of glucose-6-phosphate dehydrogenase expression in HepG2 cells using glucose-6-phosphate dehydrogenase antibody (Cat#4322, 1:2,000). Green, isotype control; red, glucose-6-phosphate dehydrogenase.
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
A synthesized peptide derived from human G6PD
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:200-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using glucose-6-phosphate dehydrogenase antibody (Cat#4322). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with glucose-6-phosphate dehydrogenase antibody (Cat#4322, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of glucose-6-phosphate dehydrogenase expression in HepG2 cells using glucose-6-phosphate dehydrogenase antibody (Cat#4322, 1:2,000). Green, isotype control; red, glucose-6-phosphate dehydrogenase.
Western blotting analysis using glucose-6-phosphate dehydrogenase antibody (Cat#4322). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with glucose-6-phosphate dehydrogenase antibody (Cat#4322, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of glucose-6-phosphate dehydrogenase expression in HepG2 cells using glucose-6-phosphate dehydrogenase antibody (Cat#4322, 1:2,000). Green, isotype control; red, glucose-6-phosphate dehydrogenase.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.001037s ]
SELECT * FROM `goods` WHERE `code` = '4322' LIMIT 1 [ RunTime:0.000656s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000593s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000467s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000589s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 5649 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000483s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000606s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 5649 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000414s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000534s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000393s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.082521s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (9074,10571,8098,4586,9519,5331,52,5003,6061,4737) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000924s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000574s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000276s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000511s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000259s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000343s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000434s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000267s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000206s ]
