Western blotting analysis using lamin B2 antibody (Cat#4379). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with lamin B2 antibody (Cat#4379, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of lamin B2 expression in HT-1080 cells using lamin B2 antibody (Cat#4379, 1:2,000). Green, isotype control; red, lamin B2.
Immunocytochemical staining of HT-1080 cells with Lamin B2 antibody (Cat#4379, 1:1,000). Nuclei were stained blue with DAPI; Lamin B2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using lamin B2 antibody (Cat#4379). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with lamin B2 antibody (Cat#4379, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of lamin B2 expression in HT-1080 cells using lamin B2 antibody (Cat#4379, 1:2,000). Green, isotype control; red, lamin B2.
Immunocytochemical staining of HT-1080 cells with Lamin B2 antibody (Cat#4379, 1:1,000). Nuclei were stained blue with DAPI; Lamin B2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using lamin B2 antibody (Cat#4379). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with lamin B2 antibody (Cat#4379, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of lamin B2 expression in HT-1080 cells using lamin B2 antibody (Cat#4379, 1:2,000). Green, isotype control; red, lamin B2.
Immunocytochemical staining of HT-1080 cells with Lamin B2 antibody (Cat#4379, 1:1,000). Nuclei were stained blue with DAPI; Lamin B2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000774s ]
SELECT * FROM `goods` WHERE `code` = '4379' LIMIT 1 [ RunTime:0.000518s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000415s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000362s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000363s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 5703 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000421s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000450s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 5703 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000274s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000392s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000252s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.063063s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (3760,4983,9728,4750,8176,5696,314,4988,8171,9481) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000836s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000474s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000226s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000483s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000224s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000333s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000404s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000225s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000247s ]
