Western blotting analysis using mTOR antibody (Cat#4607). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with mTOR antibody (Cat#4607, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with mTOR antibody (Cat#4607, 1:1,000). Nuclei were stained blue with DAPI; mTOR was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen
A synthesized peptide derived from human mTOR
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,500 Immunocytochemistry (ICC): 1:100-1:1,000
Note
This product is for research use only.
Data
Western blotting analysis using mTOR antibody (Cat#4607). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with mTOR antibody (Cat#4607, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with mTOR antibody (Cat#4607, 1:1,000). Nuclei were stained blue with DAPI; mTOR was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using mTOR antibody (Cat#4607). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with mTOR antibody (Cat#4607, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with mTOR antibody (Cat#4607, 1:1,000). Nuclei were stained blue with DAPI; mTOR was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.001894s ]
SELECT * FROM `goods` WHERE `code` = '4607' LIMIT 1 [ RunTime:0.001870s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.002235s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000882s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.001147s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 5921 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000874s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.003250s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 5921 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000417s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.003041s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000937s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.262241s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (7224,6781,5055,7321,7854,6341,8784,7160,7019,3969) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.004832s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.007028s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000277s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.003509s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000352s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.002198s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000991s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000628s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.001385s ]
