Western blotting analysis using HSF1 antibody (Cat#4720). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HSF1 antibody (Cat#4720, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of HSF1 expression in HepG2 cells using HSF1 antibody (Cat#4720, 1:2,000). Green, isotype control; red, HSF1.
Immunocytochemical staining of HepG2 cells with HSF1 antibody (Car#4720, 1:1,000). Nuclei were stained blue with DAPI; HSF1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using HSF1 antibody (Cat#4720). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HSF1 antibody (Cat#4720, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of HSF1 expression in HepG2 cells using HSF1 antibody (Cat#4720, 1:2,000). Green, isotype control; red, HSF1.
Immunocytochemical staining of HepG2 cells with HSF1 antibody (Car#4720, 1:1,000). Nuclei were stained blue with DAPI; HSF1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using HSF1 antibody (Cat#4720). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HSF1 antibody (Cat#4720, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of HSF1 expression in HepG2 cells using HSF1 antibody (Cat#4720, 1:2,000). Green, isotype control; red, HSF1.
Immunocytochemical staining of HepG2 cells with HSF1 antibody (Car#4720, 1:1,000). Nuclei were stained blue with DAPI; HSF1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
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