Western blotting analysis using epidermal growth factor receptor antibody (Cat#4796). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with epidermal growth factor receptor antibody (Cat#4796, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Epidermal growth factor receptor expression in HepG2 cells using Epidermal growth factor receptor antibody (Cat#4796, 1:2,000). Green, isotype control; red, Epidermal growth factor receptor.
Immunocytochemical staining of HepG2 cells with Epidermal growth factor receptor antibody (Cat#4796, 1:1,000). Nuclei were stained blue with DAPI; Epidermal growth factor receptor was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Medium. Scale bar, 20 μm.
Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen
Recombinant protein of human EGFR
Isotype
Mouse IgG2b
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB):1:500-1:2,500 Flow Cytometry (FCM): 1:200-1:2,000 Immunocytochemistry (ICC): 1:100-1:1,000
Note
This product is for research use only.
Data
Western blotting analysis using epidermal growth factor receptor antibody (Cat#4796). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with epidermal growth factor receptor antibody (Cat#4796, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Epidermal growth factor receptor expression in HepG2 cells using Epidermal growth factor receptor antibody (Cat#4796, 1:2,000). Green, isotype control; red, Epidermal growth factor receptor.
Immunocytochemical staining of HepG2 cells with Epidermal growth factor receptor antibody (Cat#4796, 1:1,000). Nuclei were stained blue with DAPI; Epidermal growth factor receptor was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Medium. Scale bar, 20 μm.
Western blotting analysis using epidermal growth factor receptor antibody (Cat#4796). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with epidermal growth factor receptor antibody (Cat#4796, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Epidermal growth factor receptor expression in HepG2 cells using Epidermal growth factor receptor antibody (Cat#4796, 1:2,000). Green, isotype control; red, Epidermal growth factor receptor.
Immunocytochemical staining of HepG2 cells with Epidermal growth factor receptor antibody (Cat#4796, 1:1,000). Nuclei were stained blue with DAPI; Epidermal growth factor receptor was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Medium. Scale bar, 20 μm.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.001678s ]
SELECT * FROM `goods` WHERE `code` = '4796' LIMIT 1 [ RunTime:0.003988s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.002931s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000589s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.004097s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 6102 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000340s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.002859s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 6102 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.006472s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.002160s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000297s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.197743s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (7130,5244,7338,8665,228,4260,5054,9013,216,3695) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.002076s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000412s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.001073s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.002941s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000496s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.002035s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000344s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000342s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000224s ]
