Western blotting analysis using CD45 antibody (Cat#5048). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CD45 antibody (Cat#5048, 1:10,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of CD45 expression in Raji cells using CD45 antibody (Cat#5048, 1:2,000). Green, isotype control; red, CD45.
Immunohistochemistry was performed on paraffin-embedded human tonsillitis using CD45 antibody (Cat#5048, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Western blotting analysis using CD45 antibody (Cat#5048). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CD45 antibody (Cat#5048, 1:10,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of CD45 expression in Raji cells using CD45 antibody (Cat#5048, 1:2,000). Green, isotype control; red, CD45.
Immunohistochemistry was performed on paraffin-embedded human tonsillitis using CD45 antibody (Cat#5048, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Western blotting analysis using CD45 antibody (Cat#5048). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CD45 antibody (Cat#5048, 1:10,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of CD45 expression in Raji cells using CD45 antibody (Cat#5048, 1:2,000). Green, isotype control; red, CD45.
Immunohistochemistry was performed on paraffin-embedded human tonsillitis using CD45 antibody (Cat#5048, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000823s ]
SELECT * FROM `goods` WHERE `code` = '5048' LIMIT 1 [ RunTime:0.000497s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000450s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000366s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000554s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 6224 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000423s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000543s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 6224 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000336s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000445s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000323s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.055331s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (8130,9034,9841,6524,5280,6801,4121,9438,9384,5131) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000701s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000520s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000238s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000521s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000257s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000345s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000397s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000373s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000246s ]
