Western blotting analysis using decapping mRNA 1B antibody (Cat#5060). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with decapping mRNA 1B antibody (Cat#5060, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Decapping mRNA 1B expression in HeLa cells using Decapping mRNA 1B antibody (Cat#5060, 1:1,000). Green, isotype control; red, Decapping mRNA 1B.
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
Recombinant protein of human DCP1B
Isotype
Mouse IgG1
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,500 Flow Cytometry (FCM): 1:200-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using decapping mRNA 1B antibody (Cat#5060). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with decapping mRNA 1B antibody (Cat#5060, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Decapping mRNA 1B expression in HeLa cells using Decapping mRNA 1B antibody (Cat#5060, 1:1,000). Green, isotype control; red, Decapping mRNA 1B.
Western blotting analysis using decapping mRNA 1B antibody (Cat#5060). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with decapping mRNA 1B antibody (Cat#5060, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Decapping mRNA 1B expression in HeLa cells using Decapping mRNA 1B antibody (Cat#5060, 1:1,000). Green, isotype control; red, Decapping mRNA 1B.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000893s ]
SELECT * FROM `goods` WHERE `code` = '5060' LIMIT 1 [ RunTime:0.001456s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.001367s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000564s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000834s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 6231 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.002718s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.001020s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 6231 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001099s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.001941s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000269s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.066516s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (5462,8671,6079,6039,6269,5360,9241,5794,4393,8419) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.004267s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.001733s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000228s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000448s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.002594s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001616s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000447s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000243s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.001150s ]
