Western blotting analysis using SHMT1 antibody (Cat#5130). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SHMT1 antibody (Cat#5130, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of SHMT1 expression in HepG2 cells using SHMT1 antibody (Cat#5130, 1:2,000). Green, isotype control; red, SHMT1.
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
Recombinant protein of human SHMT1
Isotype
Mouse IgG2a
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:200-1:1,000 Flow Cytometry (FCM): 1:200-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using SHMT1 antibody (Cat#5130). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SHMT1 antibody (Cat#5130, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of SHMT1 expression in HepG2 cells using SHMT1 antibody (Cat#5130, 1:2,000). Green, isotype control; red, SHMT1.
Western blotting analysis using SHMT1 antibody (Cat#5130). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SHMT1 antibody (Cat#5130, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of SHMT1 expression in HepG2 cells using SHMT1 antibody (Cat#5130, 1:2,000). Green, isotype control; red, SHMT1.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000815s ]
SELECT * FROM `goods` WHERE `code` = '5130' LIMIT 1 [ RunTime:0.001407s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000469s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001740s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000581s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 6287 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000909s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.001687s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 6287 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000985s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000791s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000537s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.130720s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (314,10435,8529,5504,10292,5784,5573,6276,10136,10316) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.006543s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.003359s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000804s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.001983s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000236s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000379s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.002154s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000246s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000229s ]
