Western blotting analysis using Cyclin B1 antibody (Cat#5151). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Cyclin B1 antibody (Cat#5151, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human tonsillitis using Cyclin B1 antibody (Cat#5151, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HepG2 cells with Cyclin B1 antibody (Cat#5151, 1:1,000). Nuclei were stained blue with DAPI; Cyclin B1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Flow cytometric analysis of Cyclin B1 expression in HepG2 cells using Cyclin B1 antibody (Cat#5151, 1:2,000). Green, isotype control; red, Cyclin B1.
Western blotting analysis using Cyclin B1 antibody (Cat#5151). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Cyclin B1 antibody (Cat#5151, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human tonsillitis using Cyclin B1 antibody (Cat#5151, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HepG2 cells with Cyclin B1 antibody (Cat#5151, 1:1,000). Nuclei were stained blue with DAPI; Cyclin B1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Flow cytometric analysis of Cyclin B1 expression in HepG2 cells using Cyclin B1 antibody (Cat#5151, 1:2,000). Green, isotype control; red, Cyclin B1.
Western blotting analysis using Cyclin B1 antibody (Cat#5151). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Cyclin B1 antibody (Cat#5151, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human tonsillitis using Cyclin B1 antibody (Cat#5151, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HepG2 cells with Cyclin B1 antibody (Cat#5151, 1:1,000). Nuclei were stained blue with DAPI; Cyclin B1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Flow cytometric analysis of Cyclin B1 expression in HepG2 cells using Cyclin B1 antibody (Cat#5151, 1:2,000). Green, isotype control; red, Cyclin B1.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000880s ]
SELECT * FROM `goods` WHERE `code` = '5151' LIMIT 1 [ RunTime:0.000446s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000467s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000322s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000441s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 6305 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000468s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000476s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 6305 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000270s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000374s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000270s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.106915s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (4032,8507,9092,6939,10307,6531,8100,4543,10379,4374) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000757s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.002394s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000229s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000458s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000212s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001074s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000599s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000299s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000283s ]
