Western blotting analysis using FRAT1 antibody (Cat#5208). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FRAT1 antibody (Cat#5208, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of FRAT1 expression in C2C12 cells using FRAT1 antibody (Cat#5208, 1:2,000). Green, isotype control; red, FRAT1.
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
A synthesized peptide derived from human FRAT1
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:100-1:1,000
Note
This product is for research use only.
Data
Western blotting analysis using FRAT1 antibody (Cat#5208). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FRAT1 antibody (Cat#5208, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of FRAT1 expression in C2C12 cells using FRAT1 antibody (Cat#5208, 1:2,000). Green, isotype control; red, FRAT1.
Western blotting analysis using FRAT1 antibody (Cat#5208). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FRAT1 antibody (Cat#5208, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of FRAT1 expression in C2C12 cells using FRAT1 antibody (Cat#5208, 1:2,000). Green, isotype control; red, FRAT1.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000636s ]
SELECT * FROM `goods` WHERE `code` = '5208' LIMIT 1 [ RunTime:0.001549s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.002098s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000305s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000379s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 6335 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000309s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000808s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 6335 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000456s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.004255s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000380s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.112963s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (5045,10259,4335,8855,6802,9018,6043,8821,8322,9856) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001083s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000772s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000263s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000509s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000397s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000536s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000625s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000375s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000299s ]
