Western blotting analysis using general transcription factor IIB antibody (Cat#5262). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with general transcription factor IIB antibody (Cat#5262, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of General transcription factor IIB expression in HeLa cells using General transcription factor IIB antibody (Cat#5262, 1:2,000). Green, isotype control; red, General transcription factor IIB.
Immunocytochemical staining of HeLa cells with General transcription factor IIB antibody (Cat#5262, 1:1,000). Nuclei were stained blue with DAPI; General transcription factor IIB was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using general transcription factor IIB antibody (Cat#5262). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with general transcription factor IIB antibody (Cat#5262, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of General transcription factor IIB expression in HeLa cells using General transcription factor IIB antibody (Cat#5262, 1:2,000). Green, isotype control; red, General transcription factor IIB.
Immunocytochemical staining of HeLa cells with General transcription factor IIB antibody (Cat#5262, 1:1,000). Nuclei were stained blue with DAPI; General transcription factor IIB was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using general transcription factor IIB antibody (Cat#5262). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with general transcription factor IIB antibody (Cat#5262, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of General transcription factor IIB expression in HeLa cells using General transcription factor IIB antibody (Cat#5262, 1:2,000). Green, isotype control; red, General transcription factor IIB.
Immunocytochemical staining of HeLa cells with General transcription factor IIB antibody (Cat#5262, 1:1,000). Nuclei were stained blue with DAPI; General transcription factor IIB was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000761s ]
SELECT * FROM `goods` WHERE `code` = '5262' LIMIT 1 [ RunTime:0.000489s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000482s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000374s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000454s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 6369 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000402s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000448s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 6369 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000392s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000419s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000253s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.052905s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (7002,6777,7147,7236,10316,9570,310,9682,4786,4221) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000861s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000498s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000325s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000470s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000251s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000421s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000462s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000261s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000235s ]
