Western blotting analysis using ferritin light chain antibody (Cat#5274). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ferritin light chain antibody (Cat#5274, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Ferritin light chain expression in HepG2 cells using Ferritin light chain antibody (Cat#5274, 1:2,000). Green, isotype control; red, Ferritin light chain.
Immunohistochemistry was performed on paraffin-embedded mouse liver using ferritin light chain antibody (Cat#5274, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Immunohistochemistry was performed on paraffin-embedded human pancreatic adenocarcinoma using ferritin light chain antibody (Cat#5274, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
