Western blotting analysis using PNP antibody (Cat#5277). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PNP antibody (Cat#5277, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of PNP expression in HepG2 cells using PNP antibody (Cat#5277, 1:2,000). Green, isotype control; red, PNP.
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
Recombinant protein of human PNP
Isotype
Mouse IgG1
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:400-1:2,000 Flow Cytometry (FCM): 1:200-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using PNP antibody (Cat#5277). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PNP antibody (Cat#5277, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of PNP expression in HepG2 cells using PNP antibody (Cat#5277, 1:2,000). Green, isotype control; red, PNP.
Western blotting analysis using PNP antibody (Cat#5277). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PNP antibody (Cat#5277, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of PNP expression in HepG2 cells using PNP antibody (Cat#5277, 1:2,000). Green, isotype control; red, PNP.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.002652s ]
SELECT * FROM `goods` WHERE `code` = '5277' LIMIT 1 [ RunTime:0.002020s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.001361s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001566s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.002124s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 6376 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000707s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.001940s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 6376 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001171s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.001515s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000577s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.181576s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (6610,7332,107,86,5604,6693,5706,4404,9714,6884) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.003140s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000727s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000336s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.003534s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000362s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000982s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.001162s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.002764s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000319s ]
