Western blotting analysis using phosphoglycerate kinase 1 antibody (Cat#5328). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phosphoglycerate kinase 1 antibody (Cat#5328, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Phosphoglycerate kinase 1 expression in HepG2 cells using Phosphoglycerate kinase 1 antibody (Cat#5328, 1:2,000). Green, isotype control; red,Phosphoglycerate kinase 1 .
Immunocytochemical staining of HepG2 cells with Phosphoglycerate kinase 1 antibody (Cat#5328, 1:1,000). Nuclei were stained blue with DAPI; Phosphoglycerate kinase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Immunohistochemistry was performed on paraffin-embedded human glioblastoma using phosphoglycerate kinase 1 antibody (Cat#5328, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
