Western blotting analysis using EZH2 antibody (Cat#5386). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with EZH2 antibody (Cat#5386, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of EZH2 expression in HAP-1 cells using EZH2 antibody (Cat#5386, 1:2,000). Green, isotype control; red, EZH2.
Immunocytochemical staining of HAP-1 cells with EZH2 antibody (Cat#5386, 1:1,000). Nuclei were stained blue with DAPI; EZH2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: High. Scale bar, 20 μm.
Western blotting analysis using EZH2 antibody (Cat#5386). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with EZH2 antibody (Cat#5386, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of EZH2 expression in HAP-1 cells using EZH2 antibody (Cat#5386, 1:2,000). Green, isotype control; red, EZH2.
Immunocytochemical staining of HAP-1 cells with EZH2 antibody (Cat#5386, 1:1,000). Nuclei were stained blue with DAPI; EZH2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: High. Scale bar, 20 μm.
Western blotting analysis using EZH2 antibody (Cat#5386). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with EZH2 antibody (Cat#5386, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of EZH2 expression in HAP-1 cells using EZH2 antibody (Cat#5386, 1:2,000). Green, isotype control; red, EZH2.
Immunocytochemical staining of HAP-1 cells with EZH2 antibody (Cat#5386, 1:1,000). Nuclei were stained blue with DAPI; EZH2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: High. Scale bar, 20 μm.
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