Western blotting analysis using DNAJA2 antibody (Cat#5407). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DNAJA2 antibody (Cat#5407, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of DNAJA2 expression in C2C12 cells using DNAJA2 antibody (Cat#5407, 1:1,000). Green, isotype control; red, DNAJA2.
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
Recombinant protein of human DNAJA2
Isotype
Mouse IgG1
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,500 Flow Cytometry (FCM): 1:100-1:1,000
Note
This product is for research use only.
Data
Western blotting analysis using DNAJA2 antibody (Cat#5407). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DNAJA2 antibody (Cat#5407, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of DNAJA2 expression in C2C12 cells using DNAJA2 antibody (Cat#5407, 1:1,000). Green, isotype control; red, DNAJA2.
Western blotting analysis using DNAJA2 antibody (Cat#5407). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DNAJA2 antibody (Cat#5407, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of DNAJA2 expression in C2C12 cells using DNAJA2 antibody (Cat#5407, 1:1,000). Green, isotype control; red, DNAJA2.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.001665s ]
SELECT * FROM `goods` WHERE `code` = '5407' LIMIT 1 [ RunTime:0.003466s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000454s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001187s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.001652s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 6493 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.003286s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.001062s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 6493 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000318s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.002886s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000737s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.210692s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (4419,4135,8175,5334,5956,7953,5446,8749,5534,6430) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001341s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.007950s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000470s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.004824s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000420s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000383s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.002384s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.001119s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.003816s ]
