Western blotting analysis using EIF4E2 antibody (Cat#5408). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with EIF4E2 antibody (Cat#5408, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of EIF4E2 expression in HepG2 cells using EIF4E2 antibody (Cat#5408, 1:1,000). Green, isotype control; red, EIF4E2.
EIF4E2; Eukaryotic Translation Initiation Factor 4E Family Member 2; Eukaryotic Translation Initiation Factor 4E-Like 3; EIF4EL3; IF4e; 4EHP; Eukaryotic Translation Initiation Factor 4E Homologous Protein; Eukaryotic Translation Initiation Factor 4E Type 2; MRNA Cap-Binding Protein Type 3; EIF4E-Like Protein 4E-LP; EIF-4E Type 2; H4EHP; EIF4E-Like Cap-Binding Protein; MRNA Cap-Binding Protein 4EHP; EIF4E Type 2; 4E-LP
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
Recombinant protein of human EIF4E2
Isotype
Mouse IgG1
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western blotting (WB): 1:500-1:2,500 Flow Cytometry (FCM): 1:100-1:1,000
Note
This product is for research use only.
Data
Western blotting analysis using EIF4E2 antibody (Cat#5408). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with EIF4E2 antibody (Cat#5408, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of EIF4E2 expression in HepG2 cells using EIF4E2 antibody (Cat#5408, 1:1,000). Green, isotype control; red, EIF4E2.
Western blotting analysis using EIF4E2 antibody (Cat#5408). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with EIF4E2 antibody (Cat#5408, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of EIF4E2 expression in HepG2 cells using EIF4E2 antibody (Cat#5408, 1:1,000). Green, isotype control; red, EIF4E2.
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