Western blotting analysis using cortactin antibody (Cat#5472). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with cortactin antibody (Cat#5472, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Cortactin expression in H9c2 cells using Cortactin antibody (Cat#5472, 1:2,000). Green, isotype control; red, Cortactin.
Immunocytochemical staining of H9C2 cells with Cortactin antibody (Cat#5472, 1:1,000). Nuclei were stained blue with DAPI; Cortactin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using cortactin antibody (Cat#5472). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with cortactin antibody (Cat#5472, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Cortactin expression in H9c2 cells using Cortactin antibody (Cat#5472, 1:2,000). Green, isotype control; red, Cortactin.
Immunocytochemical staining of H9C2 cells with Cortactin antibody (Cat#5472, 1:1,000). Nuclei were stained blue with DAPI; Cortactin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using cortactin antibody (Cat#5472). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with cortactin antibody (Cat#5472, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Cortactin expression in H9c2 cells using Cortactin antibody (Cat#5472, 1:2,000). Green, isotype control; red, Cortactin.
Immunocytochemical staining of H9C2 cells with Cortactin antibody (Cat#5472, 1:1,000). Nuclei were stained blue with DAPI; Cortactin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000794s ]
SELECT * FROM `goods` WHERE `code` = '5472' LIMIT 1 [ RunTime:0.000655s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000649s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000517s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000594s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 6541 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000527s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000533s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 6541 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000370s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000448s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000474s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.089640s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (8914,6798,9278,5604,7653,4283,5828,5467,7718,10302) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001283s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.001163s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000309s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000574s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000234s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000348s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000379s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000274s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000216s ]
