Western blotting analysis using HLF antibody (Cat#5490). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HLF antibody (Cat#5490, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of HLF expression in HepG2 cells using HLF antibody (Cat#5490, 1:2,000). Green, isotype control; red, HLF.
Immunocytochemical staining of HepG2 cells with HLF antibody (Cat#5490, 1:1,000). Nuclei were stained blue with DAPI; HLF was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
HLF; HLF Transcription Factor, PAR BZIP Family Member; Hepatic Leukemia Factor; HLF, PAR BZIP Transcription Factor; PAR BZIP Transcription Factor; MGC33822; E2A-HLF Fusion Transcript Protein
Western blotting analysis using HLF antibody (Cat#5490). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HLF antibody (Cat#5490, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of HLF expression in HepG2 cells using HLF antibody (Cat#5490, 1:2,000). Green, isotype control; red, HLF.
Immunocytochemical staining of HepG2 cells with HLF antibody (Cat#5490, 1:1,000). Nuclei were stained blue with DAPI; HLF was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using HLF antibody (Cat#5490). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HLF antibody (Cat#5490, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of HLF expression in HepG2 cells using HLF antibody (Cat#5490, 1:2,000). Green, isotype control; red, HLF.
Immunocytochemical staining of HepG2 cells with HLF antibody (Cat#5490, 1:1,000). Nuclei were stained blue with DAPI; HLF was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
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