Western blotting analysis using UBE2M antibody (Cat#5540). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with UBE2M antibody (Cat#5540, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of UBE2M expression in HepG2 cells using UBE2M antibody (Cat#5540, 1:2,000). Green, isotype control; red, UBE2M.
Immunocytochemical staining of HepG2 cells with UBE2M antibody (Cat#5540, 1:1,000). Nuclei were stained blue with DAPI; UBE2M was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using UBE2M antibody (Cat#5540). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with UBE2M antibody (Cat#5540, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of UBE2M expression in HepG2 cells using UBE2M antibody (Cat#5540, 1:2,000). Green, isotype control; red, UBE2M.
Immunocytochemical staining of HepG2 cells with UBE2M antibody (Cat#5540, 1:1,000). Nuclei were stained blue with DAPI; UBE2M was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using UBE2M antibody (Cat#5540). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with UBE2M antibody (Cat#5540, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of UBE2M expression in HepG2 cells using UBE2M antibody (Cat#5540, 1:2,000). Green, isotype control; red, UBE2M.
Immunocytochemical staining of HepG2 cells with UBE2M antibody (Cat#5540, 1:1,000). Nuclei were stained blue with DAPI; UBE2M was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.001626s ]
SELECT * FROM `goods` WHERE `code` = '5540' LIMIT 1 [ RunTime:0.001973s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000667s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000619s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.001353s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 6603 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000882s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.002252s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 6603 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000721s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000712s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000876s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.151449s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (3793,9510,6930,10161,3901,8460,6326,9853,8038,3865) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.002189s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000832s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000432s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.002550s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000225s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000452s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000345s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000412s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000491s ]
