Western blotting analysis using TRIM23 antibody (Cat#5618). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TRIM23 antibody (Cat#5618, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of TRIM23 expression in HepG2 cells using TRIM23 antibody (Cat#5618, 1:2,000). Green, isotype control; red, TRIM23.
Immunocytochemical staining of HepG2 cells with TRIM23 antibody (Cat#5618, 1:1,000). Nuclei were stained blue with DAPI; TRIM23 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using TRIM23 antibody (Cat#5618). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TRIM23 antibody (Cat#5618, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of TRIM23 expression in HepG2 cells using TRIM23 antibody (Cat#5618, 1:2,000). Green, isotype control; red, TRIM23.
Immunocytochemical staining of HepG2 cells with TRIM23 antibody (Cat#5618, 1:1,000). Nuclei were stained blue with DAPI; TRIM23 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using TRIM23 antibody (Cat#5618). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TRIM23 antibody (Cat#5618, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of TRIM23 expression in HepG2 cells using TRIM23 antibody (Cat#5618, 1:2,000). Green, isotype control; red, TRIM23.
Immunocytochemical staining of HepG2 cells with TRIM23 antibody (Cat#5618, 1:1,000). Nuclei were stained blue with DAPI; TRIM23 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.004208s ]
SELECT * FROM `goods` WHERE `code` = '5618' LIMIT 1 [ RunTime:0.000624s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.003905s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000362s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000430s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 6647 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.002201s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000583s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 6647 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.006371s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000454s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000270s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.246871s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (3777,3680,4668,4976,5141,4721,9992,9663,8536,4634) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001873s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.008453s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.001116s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000666s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.002837s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000381s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000803s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.004478s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000595s ]