Western blotting analysis using villin 1 antibody (Cat#5627). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with villin 1 antibody (Cat#5627, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:5,000
Note
This product is for research use only.
Data
Western blotting analysis using villin 1 antibody (Cat#5627). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with villin 1 antibody (Cat#5627, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using villin 1 antibody (Cat#5627). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with villin 1 antibody (Cat#5627, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.001468s ]
SELECT * FROM `goods` WHERE `code` = '5627' LIMIT 1 [ RunTime:0.000429s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000389s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.003820s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000346s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 6653 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.004449s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000444s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 6653 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000271s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000392s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000232s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.073161s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (10283,7382,9814,80,9199,8911,7675,4155,6440,8162) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000980s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000520s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000253s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000502s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000270s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000335s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000353s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000256s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000278s ]
