Western blotting analysis using IHH antibody (Cat#5646). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with IHH antibody (Cat#5646, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of IHH expression in C2C12 cells using IHH antibody (Cat#5646, 1:2,000). Green, isotype control; red, IHH.
IHH; Indian Hedgehog Signaling Molecule; HHG2; BDA1; Indian Hedgehog Protein; Indian Hedgehog (Drosophila) Homolog; Indian Hedgehog Homolog; EC 3.1.-.-; HHG-2
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
A synthesized peptide derived from human IHH
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:200-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using IHH antibody (Cat#5646). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with IHH antibody (Cat#5646, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of IHH expression in C2C12 cells using IHH antibody (Cat#5646, 1:2,000). Green, isotype control; red, IHH.
Western blotting analysis using IHH antibody (Cat#5646). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with IHH antibody (Cat#5646, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of IHH expression in C2C12 cells using IHH antibody (Cat#5646, 1:2,000). Green, isotype control; red, IHH.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000769s ]
SELECT * FROM `goods` WHERE `code` = '5646' LIMIT 1 [ RunTime:0.000511s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000495s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000334s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000532s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 6670 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000340s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000492s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 6670 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000351s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000418s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000269s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.059204s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (6432,5788,8961,7469,9390,10524,3966,6546,185,8948) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000770s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000556s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000258s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000437s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000228s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000301s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000373s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000225s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000211s ]
