Western blotting analysis using calpastatin antibody (Cat#5675). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with calpastatin antibody (Cat#5675, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,500
Note
This product is for research use only.
Data
Western blotting analysis using calpastatin antibody (Cat#5675). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with calpastatin antibody (Cat#5675, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using calpastatin antibody (Cat#5675). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with calpastatin antibody (Cat#5675, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000737s ]
SELECT * FROM `goods` WHERE `code` = '5675' LIMIT 1 [ RunTime:0.000564s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000406s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000359s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000471s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 6687 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000365s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000480s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 6687 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000323s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000410s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000350s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.061013s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (7941,186,8224,8201,9082,4949,7109,5699,9308,10243) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001031s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000535s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000277s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000457s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000273s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000308s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000387s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000243s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000199s ]
