Western blotting analysis using GALE antibody (Cat#5693). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with GALE antibody (Cat#5693, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of GALE expression in HepG2 cells using GALE antibody (Cat#5693, 1:2,000). Green, isotype control; red, GALE.
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
Recombinant protein of human GALE
Isotype
Mouse IgG1
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,500 Flow Cytometry (FCM): 1:200-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using GALE antibody (Cat#5693). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with GALE antibody (Cat#5693, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of GALE expression in HepG2 cells using GALE antibody (Cat#5693, 1:2,000). Green, isotype control; red, GALE.
Western blotting analysis using GALE antibody (Cat#5693). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with GALE antibody (Cat#5693, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of GALE expression in HepG2 cells using GALE antibody (Cat#5693, 1:2,000). Green, isotype control; red, GALE.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000800s ]
SELECT * FROM `goods` WHERE `code` = '5693' LIMIT 1 [ RunTime:0.000544s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000464s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000344s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000440s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 6705 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000452s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000459s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 6705 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000330s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000449s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000266s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.055016s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (6548,10499,6223,4132,5960,5407,9303,7275,8077,5935) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000803s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000480s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000234s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000501s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000243s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000300s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000373s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000252s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000204s ]
