Western blotting analysis using GDI1 antibody (Cat#5698). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with GDI1 antibody (Cat#5698, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human breast carcinoma using GDI1 antibody (Cat#5698, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of GDI1 expression in HAP-1 cells using GDI1 antibody (Cat#5698, 1:2,000). Green, isotype control; red, GDI1.
Western blotting analysis using GDI1 antibody (Cat#5698). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with GDI1 antibody (Cat#5698, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human breast carcinoma using GDI1 antibody (Cat#5698, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of GDI1 expression in HAP-1 cells using GDI1 antibody (Cat#5698, 1:2,000). Green, isotype control; red, GDI1.
Western blotting analysis using GDI1 antibody (Cat#5698). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with GDI1 antibody (Cat#5698, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human breast carcinoma using GDI1 antibody (Cat#5698, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of GDI1 expression in HAP-1 cells using GDI1 antibody (Cat#5698, 1:2,000). Green, isotype control; red, GDI1.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.002379s ]
SELECT * FROM `goods` WHERE `code` = '5698' LIMIT 1 [ RunTime:0.000578s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.001215s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000488s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.001006s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 6710 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000794s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.004470s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 6710 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001753s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000482s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000277s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.090840s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (9143,5083,9182,4686,9346,7599,7928,7141,8078,332) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001114s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000445s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000240s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.001860s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000955s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000363s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000412s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000820s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000198s ]
