Western blotting analysis using FUBP3 antibody (Cat#5722). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FUBP3 antibody (Cat#5722, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HT-1080 cells with FUBP3 antibody (Cat#5722, 1:1,000). Nuclei were stained blue with DAPI; FUBP3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Flow cytometric analysis of FUBP3 expression in HT-1080 cells using FUBP3 antibody (Cat#5722, 1:2,000). Green, isotype control; red, FUBP3.
FUBP3; Far Upstream Element Binding Protein 3; FBP3; Far Upstream Element (FUSE) Binding Protein 3; Far Upstream Element-Binding Protein 3; FUSE-Binding Protein 3
Western blotting analysis using FUBP3 antibody (Cat#5722). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FUBP3 antibody (Cat#5722, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HT-1080 cells with FUBP3 antibody (Cat#5722, 1:1,000). Nuclei were stained blue with DAPI; FUBP3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Flow cytometric analysis of FUBP3 expression in HT-1080 cells using FUBP3 antibody (Cat#5722, 1:2,000). Green, isotype control; red, FUBP3.
Western blotting analysis using FUBP3 antibody (Cat#5722). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FUBP3 antibody (Cat#5722, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HT-1080 cells with FUBP3 antibody (Cat#5722, 1:1,000). Nuclei were stained blue with DAPI; FUBP3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Flow cytometric analysis of FUBP3 expression in HT-1080 cells using FUBP3 antibody (Cat#5722, 1:2,000). Green, isotype control; red, FUBP3.
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