Western blotting analysis using PRKCE antibody (Cat#5846). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PRKCE antibody (Cat#5846, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of RH30 cells with PRKCE antibody (Cat#5846, 1:1,000). Nuclei were stained blue with DAPI; PRKCE was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Flow cytometric analysis of PRKCE expression in RH30 cells using PRKCE antibody (Cat#5846, 1:2,000). Green, isotype control; red, PRKCE.
Western blotting analysis using PRKCE antibody (Cat#5846). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PRKCE antibody (Cat#5846, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of RH30 cells with PRKCE antibody (Cat#5846, 1:1,000). Nuclei were stained blue with DAPI; PRKCE was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Flow cytometric analysis of PRKCE expression in RH30 cells using PRKCE antibody (Cat#5846, 1:2,000). Green, isotype control; red, PRKCE.
Western blotting analysis using PRKCE antibody (Cat#5846). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PRKCE antibody (Cat#5846, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of RH30 cells with PRKCE antibody (Cat#5846, 1:1,000). Nuclei were stained blue with DAPI; PRKCE was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Flow cytometric analysis of PRKCE expression in RH30 cells using PRKCE antibody (Cat#5846, 1:2,000). Green, isotype control; red, PRKCE.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000791s ]
SELECT * FROM `goods` WHERE `code` = '5846' LIMIT 1 [ RunTime:0.000497s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000533s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000379s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000400s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 6845 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000379s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000438s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 6845 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000274s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000367s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000293s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.054396s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (4090,8052,9223,6537,8130,4694,6875,8220,5811,80) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000831s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000456s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000237s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000473s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000257s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000322s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000437s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000303s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000245s ]
