Western blotting analysis using HPGD antibody (Cat#5847). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HPGD antibody (Cat#5847, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of HPGD expression in A549 cells using HPGD antibody (Cat#5847, 1:2,000). Green, isotype control; red, HPGD.
Immunocytochemical staining of A549 cells with HPGD antibody(Cat#5847, 1:1,000). Nuclei were stained blue with DAPI; HPGD was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using HPGD antibody (Cat#5847). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HPGD antibody (Cat#5847, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of HPGD expression in A549 cells using HPGD antibody (Cat#5847, 1:2,000). Green, isotype control; red, HPGD.
Immunocytochemical staining of A549 cells with HPGD antibody(Cat#5847, 1:1,000). Nuclei were stained blue with DAPI; HPGD was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using HPGD antibody (Cat#5847). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HPGD antibody (Cat#5847, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of HPGD expression in A549 cells using HPGD antibody (Cat#5847, 1:2,000). Green, isotype control; red, HPGD.
Immunocytochemical staining of A549 cells with HPGD antibody(Cat#5847, 1:1,000). Nuclei were stained blue with DAPI; HPGD was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000900s ]
SELECT * FROM `goods` WHERE `code` = '5847' LIMIT 1 [ RunTime:0.000703s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000421s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.002542s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000687s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 6846 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.003254s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.004411s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 6846 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000339s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000562s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.001753s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.162318s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (9395,10531,83,36,6172,4747,10290,5367,5887,9121) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001791s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.002418s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000218s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000482s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000226s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001057s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000397s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.001161s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000233s ]
