Western blotting analysis using ANAPC7 antibody (Cat#5883). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ANAPC7 antibody (Cat#5883, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with ANAPC7 antibody (Cat#5883, 1:1,000) . Nuclei were stained blue with DAPI; ANAPC7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Flow cytometric analysis of ANAPC7 expression in HepG2 cells using ANAPC7 antibody (Cat#5883,1:2,000). Green, isotype control; red, ANAPC7.
Western blotting analysis using ANAPC7 antibody (Cat#5883). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ANAPC7 antibody (Cat#5883, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with ANAPC7 antibody (Cat#5883, 1:1,000) . Nuclei were stained blue with DAPI; ANAPC7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Flow cytometric analysis of ANAPC7 expression in HepG2 cells using ANAPC7 antibody (Cat#5883,1:2,000). Green, isotype control; red, ANAPC7.
Western blotting analysis using ANAPC7 antibody (Cat#5883). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ANAPC7 antibody (Cat#5883, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with ANAPC7 antibody (Cat#5883, 1:1,000) . Nuclei were stained blue with DAPI; ANAPC7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Flow cytometric analysis of ANAPC7 expression in HepG2 cells using ANAPC7 antibody (Cat#5883,1:2,000). Green, isotype control; red, ANAPC7.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.001324s ]
SELECT * FROM `goods` WHERE `code` = '5883' LIMIT 1 [ RunTime:0.000927s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000636s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000610s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000669s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 6864 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000479s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000655s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 6864 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000355s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000579s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000390s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.093094s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (5361,3942,8056,8747,9295,5704,6805,5069,8147,9336) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000887s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000710s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000384s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000755s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000351s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000560s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000611s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000417s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000329s ]
