Western blotting analysis using TUBB4A antibody (Cat#5895). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TUBB4A antibody (Cat#5895, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of TUBB4A expression in HAP-1 cells using TUBB4A antibody (Cat#5895, 1:2,000). Green, isotype control; red, TUBB4A.
Immunocytochemical staining of HAP-1 cells with TUBB4A antibody(Cat#5895, 1:1,000). Nuclei were stained blue with DAPI; TUBB4A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using TUBB4A antibody (Cat#5895). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TUBB4A antibody (Cat#5895, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of TUBB4A expression in HAP-1 cells using TUBB4A antibody (Cat#5895, 1:2,000). Green, isotype control; red, TUBB4A.
Immunocytochemical staining of HAP-1 cells with TUBB4A antibody(Cat#5895, 1:1,000). Nuclei were stained blue with DAPI; TUBB4A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using TUBB4A antibody (Cat#5895). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TUBB4A antibody (Cat#5895, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of TUBB4A expression in HAP-1 cells using TUBB4A antibody (Cat#5895, 1:2,000). Green, isotype control; red, TUBB4A.
Immunocytochemical staining of HAP-1 cells with TUBB4A antibody(Cat#5895, 1:1,000). Nuclei were stained blue with DAPI; TUBB4A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.001171s ]
SELECT * FROM `goods` WHERE `code` = '5895' LIMIT 1 [ RunTime:0.000587s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000612s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000492s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000432s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 6872 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000523s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000506s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 6872 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000413s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000472s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000462s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.062000s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (8006,8946,4442,10238,9808,3842,6777,3916,9388,6524) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000876s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000605s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000295s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000659s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000334s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000463s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000563s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000327s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000282s ]
