Western blotting analysis using TLE1 antibody (Cat#5908). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TLE1 antibody (Cat#5908, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of Hela cells with TLE1 antibody (Cat#5908, 1:1,000) . Nuclei were stained blue with DAPI; TLE1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
TLE1; TLE Family Member 1, Transcriptional Corepressor; ESG1; TLE-1; GRG1; ESG; Transducin-Like Enhancer Of Split 1 (E(Sp1) Homolog, Drosophila); Enhancer Of Split Groucho-Like Protein 1; Transducin Like Enhancer Of Split 1; Transducin-Like Enhancer Protein 1; Enhancer Of Split Groucho 1; E(Sp1) Homolog
Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen
Recombinant protein of human TLE1
Isotype
Mouse IgG2b
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,500 Immunocytochemistry (ICC): 1:100-1:1,000
Note
This product is for research use only.
Data
Western blotting analysis using TLE1 antibody (Cat#5908). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TLE1 antibody (Cat#5908, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of Hela cells with TLE1 antibody (Cat#5908, 1:1,000) . Nuclei were stained blue with DAPI; TLE1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using TLE1 antibody (Cat#5908). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TLE1 antibody (Cat#5908, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of Hela cells with TLE1 antibody (Cat#5908, 1:1,000) . Nuclei were stained blue with DAPI; TLE1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.001841s ]
SELECT * FROM `goods` WHERE `code` = '5908' LIMIT 1 [ RunTime:0.000647s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000437s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000356s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000372s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 6882 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000321s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000510s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 6882 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000270s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000391s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000289s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.057568s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (5829,9026,4768,6018,7904,5853,8382,7634,6131,5963) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000910s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000523s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000291s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000589s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000319s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000399s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000442s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000359s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000307s ]
