Western blotting analysis using SEC23B antibody (Cat#5925). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SEC23B antibody (Cat#5925, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of SEC23B expression in C2C12 cells using SEC23B antibody (Cat#5925, 1:2,000). Green, isotype control; red, SEC23B.
SEC23B; SEC23 Homolog B, COPII Coat Complex Component; CDA-II; HEMPAS; CDAII; Protein Transport Protein Sec23B; SEC23-Related Protein B; HSec23B; CDAN2; Congenital Dyserythropoietic Anemia, Type II; SEC23 Homolog B, Coat Complex II Component; Sec23 (S. Cerevisiae) Homolog B; Sec23 Homolog B (S. Cerevisiae); Transport Protein SEC23B; SEC23-Like Protein B; CWS7
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
Recombinant protein of human SEC23B
Isotype
Mouse IgG1
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,500 Flow Cytometry (FCM): 1:200-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using SEC23B antibody (Cat#5925). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SEC23B antibody (Cat#5925, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of SEC23B expression in C2C12 cells using SEC23B antibody (Cat#5925, 1:2,000). Green, isotype control; red, SEC23B.
Western blotting analysis using SEC23B antibody (Cat#5925). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SEC23B antibody (Cat#5925, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of SEC23B expression in C2C12 cells using SEC23B antibody (Cat#5925, 1:2,000). Green, isotype control; red, SEC23B.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000772s ]
SELECT * FROM `goods` WHERE `code` = '5925' LIMIT 1 [ RunTime:0.000497s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000525s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000436s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000565s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 6891 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000485s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000497s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 6891 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000401s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000454s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000309s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.054520s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (5949,7355,5341,5030,10241,4389,4360,5817,9660,7845) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000825s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000612s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000266s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000557s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000295s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000354s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000452s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000293s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000260s ]
