Western blotting analysis using TTLL12 antibody (Cat#5985). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TTLL12 antibody (Cat#5985, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
TTLL12; Tubulin Tyrosine Ligase Like 12; KIAA0153; Inactive Tubulin--Tyrosine Ligase-Like Protein 12; Tubulin--Tyrosine Ligase-Like Protein 12; Tubulin Tyrosine Ligase-Like Family, Member 12; Tubulin Tyrosine Ligase-Like Family Member 12; DJ526I14.2
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,500
Note
This product is for research use only.
Data
Western blotting analysis using TTLL12 antibody (Cat#5985). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TTLL12 antibody (Cat#5985, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using TTLL12 antibody (Cat#5985). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TTLL12 antibody (Cat#5985, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.002104s ]
SELECT * FROM `goods` WHERE `code` = '5985' LIMIT 1 [ RunTime:0.001859s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.001290s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000708s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.001138s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 6928 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001233s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000572s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 6928 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000796s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000797s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000729s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.090890s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (9931,5104,6154,5238,4377,7680,7267,5483,7932,7078) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000905s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000451s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.002265s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000498s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000239s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.002424s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000401s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000255s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000206s ]
