Western blotting analysis using laminin subunit gamma 1 antibody (Cat#61123). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with laminin subunit gamma 1 antibody (Cat#61123, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using laminin subunit γ 1 antibody (Cat#61123). Laminin subunit γ 1 expression in wild type (WT) and laminin subunit γ 1 (LAMC1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with laminin subunit γ 1 antibody (Cat#61123, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of laminin subunit gamma 1 expression in HAP-1 cells using laminin subunit gamma 1 antibody (Cat#61123, 1:2,000). Green, isotype control; red, laminin subunit gamma 1.