Western blotting analysis using MCM6 antibody (Cat#61136). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MCM6 antibody (Cat#61136, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using MCM6 antibody (Cat#61136). MCM6 expression in wild type (WT) and MCM6 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with MCM6 antibody (Cat#61136, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of MCM6 expression in C2C12 cells using MCM6 antibody (Cat#61136, 1:2,000). Green, isotype control; red, MCM6.
Validation of MCM6 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with MCM6 antibody (Cat#61136, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of C2C12 cells with MCM6 antibody (Cat#61136, 1:1,000). Nuclei were stained blue with DAPI; MCM6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Immunocytochemical staining of HeLa cells using MCM6 antibody (Cat#61136, 1:1,000), Top panel: wild-type (WT); Bottom panal: MCM6 shRNA knockdown (KD). Nuclei were stained blue with DAPI; MCM6 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma
using MCM6 antibody (Cat#61136, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
