Western blotting analysis using BAX antibody (Cat#61220). Total cell lysates (10 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BAX antibody (Cat#61220, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using BAX antibody (Cat#61220). BAX expression in wild type (WT) and BAX knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with BAX antibody (Cat#61220, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of BAX expression in HT-1080 cells using BAX antibody (Cat# 61220, 1:2,000). Green, isotype control; red, BAX.
Immunocytochemical staining of HT-1080 cells with BAX antibody (Cat#61220, 1:1,000). Nuclei were stained blue with DAPI; BAX was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Immunohistochemistry was performed on paraffin-embedded mouse kidney using BAX antibody (Cat#61220, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using BAX antibody (Cat#61220, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
