Western blotting analysis using thioredoxin domain containing 5 antibody (Cat#61260). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with thioredoxin domain containing 5 antibody (Cat#61260, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using thioredoxin domain containing 5 antibody (Cat#61260). Thioredoxin domain containing 5 expression in wild type (WT) and thioredoxin domain containing 5 (TXNDC5) knockdown (KD) HSHC cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with thioredoxin domain containing 5 antibody (Cat#61260, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Thioredoxin domain containing 5 expression in HepG2 cells using Thioredoxin domain containing 5 antibody (Cat# 61260, 1:2,000). Green, isotype control; red, Thioredoxin domain containing 5.
Immunocytochemical staining of HepG2 cells with Thioredoxin domain containing 5 antibody (Cat#61260, 1:1,000). Nuclei were stained blue with DAPI; Thioredoxin domain containing 5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain：Low. Scale bar, 20 μm.
