Western blotting analysis using FBXO11 antibody (Cat#61262). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FBXO11 antibody (Cat#61262, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using FBXO11 antibody (Cat#61262). FBXO11 expression in wild-type (WT) and FBXO11 knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with FBXO11 antibody (Cat#61262, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of FBXO11 expression in HeLa cells using FBXO11 antibody (Cat# 61262, 1:2,000). Green, isotype control; red, FBXO11.
FBXO11; F-Box Protein 11; FBX11; F-Box Only Protein 11; UBR6; Ubiquitin Protein Ligase E3 Component N-Recognin 6; Protein Arginine N-Methyltransferase 9; Vitiligo-Associated Protein 1; PRMT9; VIT1; Vitiligo-Associated Protein VIT-1; UG063H01; IDDFBA; VIT-1
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
Recombinant protein of human FBXO11
Isotype
Mouse IgG1
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:2,500-1:5,000 Flow Cytometry (FCM): 1:200-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using FBXO11 antibody (Cat#61262). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FBXO11 antibody (Cat#61262, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using FBXO11 antibody (Cat#61262). FBXO11 expression in wild-type (WT) and FBXO11 knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with FBXO11 antibody (Cat#61262, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of FBXO11 expression in HeLa cells using FBXO11 antibody (Cat# 61262, 1:2,000). Green, isotype control; red, FBXO11.
Western blotting analysis using FBXO11 antibody (Cat#61262). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FBXO11 antibody (Cat#61262, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using FBXO11 antibody (Cat#61262). FBXO11 expression in wild-type (WT) and FBXO11 knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with FBXO11 antibody (Cat#61262, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of FBXO11 expression in HeLa cells using FBXO11 antibody (Cat# 61262, 1:2,000). Green, isotype control; red, FBXO11.
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