Western blotting analysis using ACSL4 antibody (Cat#61331). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ACSL4 antibody (Cat#61331, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). ACSL4, acyl-CoA synthetase long chain family member 4.
Western blotting analysis using ACSL4 antibody (Cat#61331). ACSL4 expression in wild type (WT) and ACSL4 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with ACSL4 antibody (Cat#61331, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of ACSL4 expression in HT-1080 cells using ACSL4 antibody (Cat#61331, 1:2,000). Green, isotype control; red, ACSL4.
Immunocytochemical staining of HT-1080 cells with ACSL4 antibody (Cat#61331, 1:1,000). Nuclei were stained blue with DAPI; ACSL4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Immunohistochemistry was performed on paraffin-embedded mouse kidney using ACSL4 antibody (Cat#61331, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Immunohistochemistry was performed on paraffin-embedded human hepatocarcinoma using ACSL4 antibody (Cat#61331, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
