Western blotting analysis using Bridging integrator 1 antibody (Cat#61357). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Bridging integrator 1 antibody (Cat#61357, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using bridging integrator 1 antibody (Cat#61357). Bridging integrator 1 expression in wild type (WT) and bridging integrator 1 (BIN1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with bridging integrator 1 antibody (Cat#61357, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Bridging integrator 1 expression in HepG2 cells using Bridging integrator 1 antibody (Cat#61357, 1:2,000). Green, isotype control; red, Bridging integrator 1.
Validation of Bridging integrator 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Bridging integrator 1 antibody (Cat#61357, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HepG2 cells with Bridging integrator 1 antibody (Cat#61357, 1:1,000). Nuclei were stained blue with DAPI; Bridging integrator 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Immunocytochemical staining of HeLa cells using Bridging integrator 1 antibody (Cat#61357, 1:1,000), Top panel: wild-type (WT); Bottom panal: Bridging integrator 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Bridging integrator 1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
