Western blotting analysis using hippocalcin antibody (Cat#61428). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with hippocalcin antibody (Cat#61428, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using hippocalcin antibody (Cat#61428). Hippocalcin expression in wild-type (WT) and hippocalcin (HPCA) knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with hippocalcin antibody (Cat#61428, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Hippocalcin expression in HepG2 cells using Hippocalcin antibody (Cat#61428, 1:2,000). Green, isotype control; red, Hippocalcin.
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
A synthesized peptide derived from human Hippocalcin
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:200-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using hippocalcin antibody (Cat#61428). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with hippocalcin antibody (Cat#61428, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using hippocalcin antibody (Cat#61428). Hippocalcin expression in wild-type (WT) and hippocalcin (HPCA) knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with hippocalcin antibody (Cat#61428, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Hippocalcin expression in HepG2 cells using Hippocalcin antibody (Cat#61428, 1:2,000). Green, isotype control; red, Hippocalcin.
Western blotting analysis using hippocalcin antibody (Cat#61428). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with hippocalcin antibody (Cat#61428, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using hippocalcin antibody (Cat#61428). Hippocalcin expression in wild-type (WT) and hippocalcin (HPCA) knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with hippocalcin antibody (Cat#61428, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Hippocalcin expression in HepG2 cells using Hippocalcin antibody (Cat#61428, 1:2,000). Green, isotype control; red, Hippocalcin.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.002873s ]
SELECT * FROM `goods` WHERE `code` = '61428' LIMIT 1 [ RunTime:0.001490s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.001847s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001337s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.001351s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 7328 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001901s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.001646s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 7328 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000832s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.001139s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000571s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.099646s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (9042,3871,9397,6519,10201,5468,5994,7835,8864,5119) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000907s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000581s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000306s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000545s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000305s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000385s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000475s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000325s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000283s ]
