Western blotting analysis using myelin associated glycoprotein antibody (Cat#61466). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with myelin associated glycoprotein antibody (Cat#61466, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using myelin associated glycoprotein antibody (Cat#61466). Myelin associated glycoprotein expression in wild type (WT) and myelin associated glycoprotein (MAG) shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with myelin associated glycoprotein antibody (Cat#61466, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Myelin associated glycoprotein expression in HeLa cells using Myelin associated glycoprotein antibody (Cat#61466, 1:2,000). Green, isotype control; red, Myelin associated glycoprotein.
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
A synthesized peptide derived from human MAG
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:200-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using myelin associated glycoprotein antibody (Cat#61466). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with myelin associated glycoprotein antibody (Cat#61466, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using myelin associated glycoprotein antibody (Cat#61466). Myelin associated glycoprotein expression in wild type (WT) and myelin associated glycoprotein (MAG) shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with myelin associated glycoprotein antibody (Cat#61466, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Myelin associated glycoprotein expression in HeLa cells using Myelin associated glycoprotein antibody (Cat#61466, 1:2,000). Green, isotype control; red, Myelin associated glycoprotein.
Western blotting analysis using myelin associated glycoprotein antibody (Cat#61466). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with myelin associated glycoprotein antibody (Cat#61466, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using myelin associated glycoprotein antibody (Cat#61466). Myelin associated glycoprotein expression in wild type (WT) and myelin associated glycoprotein (MAG) shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with myelin associated glycoprotein antibody (Cat#61466, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Myelin associated glycoprotein expression in HeLa cells using Myelin associated glycoprotein antibody (Cat#61466, 1:2,000). Green, isotype control; red, Myelin associated glycoprotein.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.001072s ]
SELECT * FROM `goods` WHERE `code` = '61466' LIMIT 1 [ RunTime:0.000695s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000643s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000505s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000571s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 7363 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000503s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000629s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 7363 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000415s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000564s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000412s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.089519s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (6378,4506,5608,8154,7741,4842,7737,9209,8388,10576) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000934s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000563s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000283s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000618s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000270s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000436s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000495s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000355s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000253s ]
