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  • Primary Antibody
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Home>Product>Primary Antibody

KD-Validated LDL Receptor Related Protein 8 Recombinant Rabbit mAb

#61482

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using LDL receptor related protein 8 antibody (Cat#61482). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with LDL receptor related protein 8 antibody (Cat#61482, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using LDL receptor related protein 8 antibody (Cat#61482). LDL receptor related protein 8 expression in wild type (WT) and LDL receptor related protein 8 (LRP8) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with LDL receptor related protein 8 antibody (Cat#61482, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded mouse brain using LDL receptor related protein 8 antibody (Cat#61482, 1:200). Antigen retrieval was done in EDTA buffer (pH 9.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HepG2 cells with LDL receptor related protein 8 antibody (Cat#61482, 1:1,000). Nuclei were stained blue with DAPI; LDL receptor related protein 8 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Immunocytochemical staining of Hela cells using LDL receptor related protein 8 antibody (Cat#61482, 1:1,000), Top panel: wild-type (WT); Bottom panal: LDL receptor related protein 8 shRNA knockdown (KD). Nuclei were stained blue with DAPI; LDL receptor related protein 8 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
Flow cytometric analysis of LDL receptor related protein 8 expression in HepG2 cells using LDL receptor related protein 8 antibody (Cat#61482, 1:2,000). Green, isotype control; red, LDL receptor related protein 8.
Validation of LDL receptor related protein 8 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with LDL receptor related protein 8 antibody (Cat#61482, 1:2,000) and analyzed using BD flow cytometer.
Please select the specifications of the product.
Specification：
20 μL 100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    LRP8
  • Catalog：
    61482
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated LDL Receptor Related Protein 8 Recombinant Rabbit mAb
Aliases LRP8; LDL Receptor Related Protein 8; APOER2; LRP-8; HSZ75190; MCI1; Low Density Lipoprotein Receptor-Related Protein 8, Apolipoprotein E Receptor; Low-Density Lipoprotein Receptor-Related Protein 8; Apolipoprotein E Receptor 2; ApoE Receptor 2
Background

Gene Name: LRP8

NCBI Gene Entry: 7804

UniProt Entry: Q14114

Application Information

Molecular Weight: Predicted, 106 kDa, observed, 106 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB2520

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC), immunohistochemistry-paraffin (IHC-P)
Immunogen A synthesized peptide derived from human ApoER2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for 1 year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Western blotting analysis using LDL receptor related protein 8 antibody (Cat#61482). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with LDL receptor related protein 8 antibody (Cat#61482, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using LDL receptor related protein 8 antibody (Cat#61482). LDL receptor related protein 8 expression in wild type (WT) and LDL receptor related protein 8 (LRP8) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with LDL receptor related protein 8 antibody (Cat#61482, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunohistochemistry was performed on paraffin-embedded mouse brain using LDL receptor related protein 8 antibody (Cat#61482, 1:200). Antigen retrieval was done in EDTA buffer (pH 9.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Immunocytochemical staining of HepG2 cells with LDL receptor related protein 8 antibody (Cat#61482, 1:1,000). Nuclei were stained blue with DAPI; LDL receptor related protein 8 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Immunocytochemical staining of Hela cells using LDL receptor related protein 8 antibody (Cat#61482, 1:1,000), Top panel: wild-type (WT); Bottom panal: LDL receptor related protein 8 shRNA knockdown (KD). Nuclei were stained blue with DAPI; LDL receptor related protein 8 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
  • Flow cytometric analysis of LDL receptor related protein 8 expression in HepG2 cells using LDL receptor related protein 8 antibody (Cat#61482, 1:2,000). Green, isotype control; red, LDL receptor related protein 8.
  • Validation of LDL receptor related protein 8 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with LDL receptor related protein 8 antibody (Cat#61482, 1:2,000) and analyzed using BD flow cytometer.
Please select the specifications of the product.
Specification：
20 μL,100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    LRP8
  • Catalog：
    61482
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using LDL receptor related protein 8 antibody (Cat#61482). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with LDL receptor related protein 8 antibody (Cat#61482, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using LDL receptor related protein 8 antibody (Cat#61482). LDL receptor related protein 8 expression in wild type (WT) and LDL receptor related protein 8 (LRP8) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with LDL receptor related protein 8 antibody (Cat#61482, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded mouse brain using LDL receptor related protein 8 antibody (Cat#61482, 1:200). Antigen retrieval was done in EDTA buffer (pH 9.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HepG2 cells with LDL receptor related protein 8 antibody (Cat#61482, 1:1,000). Nuclei were stained blue with DAPI; LDL receptor related protein 8 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Immunocytochemical staining of Hela cells using LDL receptor related protein 8 antibody (Cat#61482, 1:1,000), Top panel: wild-type (WT); Bottom panal: LDL receptor related protein 8 shRNA knockdown (KD). Nuclei were stained blue with DAPI; LDL receptor related protein 8 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
Flow cytometric analysis of LDL receptor related protein 8 expression in HepG2 cells using LDL receptor related protein 8 antibody (Cat#61482, 1:2,000). Green, isotype control; red, LDL receptor related protein 8.
Validation of LDL receptor related protein 8 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with LDL receptor related protein 8 antibody (Cat#61482, 1:2,000) and analyzed using BD flow cytometer.
Shopping cart (12)
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Estimated total order price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
Subtotal：$23400
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  • Email Address

    support@genuinbiotech.com

    sales@genuinbiotech.com

  • Office Hours

    8:30 AM - 5:30 PM EST Mon-Fri

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  126. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-orm/src/db/concern/TimeFieldQuery.php ( 7.43 KB )
  127. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-orm/src/db/concern/AggregateQuery.php ( 3.26 KB )
  128. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-orm/src/db/concern/ModelRelationQuery.php ( 20.07 KB )
  129. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-orm/src/db/concern/ParamsBind.php ( 3.66 KB )
  130. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-orm/src/db/concern/ResultOperation.php ( 7.01 KB )
  131. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-orm/src/db/concern/WhereQuery.php ( 19.37 KB )
  132. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-orm/src/db/concern/JoinAndViewQuery.php ( 7.11 KB )
  133. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-orm/src/db/concern/TableFieldInfo.php ( 2.63 KB )
  134. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-orm/src/db/concern/Transaction.php ( 2.77 KB )
  135. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/log/driver/File.php ( 5.96 KB )
  136. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/contract/LogHandlerInterface.php ( 0.86 KB )
  137. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/log/Channel.php ( 3.89 KB )
  138. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/event/LogRecord.php ( 1.02 KB )
  139. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-helper/src/Collection.php ( 16.47 KB )
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  145. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/public.php ( 3.56 KB )
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  1. CONNECT:[ UseTime:0.000511s ] mysql:host=127.0.0.1;port=3306;dbname=sbywcs_dsger_com;charset=utf8mb4
  2. SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000828s ]
  3. SELECT * FROM `goods` WHERE `code` = '61482' LIMIT 1 [ RunTime:0.000487s ]
  4. SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000403s ]
  5. SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000388s ]
  6. SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000363s ]
  7. SELECT * FROM `goods_album` WHERE `goods_id` = 7377 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000378s ]
  8. SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000463s ]
  9. SELECT * FROM `goods_quotation` WHERE `goods_id` = 7377 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000407s ]
  10. SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000396s ]
  11. SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000252s ]
  12. SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.054135s ]
  13. SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (8428,5101,5733,3756,9417,7628,7696,7143,5395,9545) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000881s ]
  14. SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000571s ]
  15. SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000255s ]
  16. SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000428s ]
  17. SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000234s ]
  18. SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000308s ]
  19. SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000370s ]
  20. SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000262s ]
  21. SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000209s ]
0.092508s