Flow cytometric analysis of Cytochrome b5 reductase 3 expression in H9c2 cells using Cytochrome b5 reductase 3 antibody (Cat#61552, 1:2,000). Green, isotype control; red, Cytochrome b5 reductase 3.
Immunocytochemical staining of H9c2 cells with Cytochrome b5 reductase 3 antibody (Cat#61552, 1:1,000). Nuclei were stained blue with DAPI; Cytochrome b5 reductase 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Immunohistochemistry was performed on paraffin-embedded human glioblastoma using cytochrome b5 reductase 3 antibody (Cat#61552, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Western blotting analysis using cytochrome b5 reductase 3 antibody (Cat#61552). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with cytochrome b5 reductase 3 antibody (Cat#61552, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using cytochrome b5 reductase 3 antibody (Cat#61552). Cytochrome b5 reductase 3 expression in wild type (WT) and cytochrome b5 reductase 3 (CYB5R3) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with cytochrome b5 reductase 3 antibody (Cat#61552, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
