Western blotting analysis using TBP antibody (Cat#6156). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TBP antibody (Cat#6156, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with TBP antibody (Cat#6156, 1:1,000). Nuclei were stained blue with DAPI; TBP was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Applications Tested: Western Blotting (WB), immunocytochemistry (ICC)
Immunogen
Purified protein of human TBP
Isotype
Mouse IgG1
Storage Buffer
Supplied in PBS (pH 7.4) containing 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:400-1:2,000 Immunocytochemistry (ICC): 1:100-1:1,000
Note
This product is for research use only.
Data
Western blotting analysis using TBP antibody (Cat#6156). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TBP antibody (Cat#6156, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with TBP antibody (Cat#6156, 1:1,000). Nuclei were stained blue with DAPI; TBP was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using TBP antibody (Cat#6156). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TBP antibody (Cat#6156, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with TBP antibody (Cat#6156, 1:1,000). Nuclei were stained blue with DAPI; TBP was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000705s ]
SELECT * FROM `goods` WHERE `code` = '6156' LIMIT 1 [ RunTime:0.000529s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000483s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000406s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000478s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 9955 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000419s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000480s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 9955 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000357s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000451s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000409s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.052162s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (4266,9141,7703,8024,9220,8148,4357,5083,9207,8064) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000870s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000458s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000231s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000589s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000268s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000319s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000440s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000281s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000272s ]
